1. Overview of Parasitological Examination of
- Stool
- Urine
- Sputum
- Other body fluids and biopsies
2. Serological applications of diagnostic parasitology
At our practical, we had a hands-on experience of an Enzyme Linked Immunosorbent Assay (ELISA) for the detection of Toxoplasma IgM antibodies in human serum.
WHAT ARE SOME OF THE CAUSES OF FALSE POSITIVE AND FALSE NEGATIVE IN THE ELISA TEST?
ReplyDeleteCauses of false positive and false negative in ELISA
Delete1. Wrong pipetting
2. Improper washing
3. Wrong timing for incubation especially the substrate (TMB) part. If its under-timed it will affect it and if its over-timed you may get a lot of false color formations.
4. If after washing, you don't drain-off the washing solution from wells completely, it will also affect your results
5. Leaving bubbles in your wells after washing will also affect it.
6. On the average, you should read your results on the photometer within the first 10minutes after adding your stop solution. Remember that some kits will recommend that after adding the stop solution, you should wait for some minutes before reading your results. Gentle agitation of the plate will also ensure uniform color formation for reading.
7. Leaving water or other solutions under u plate will also affect your reading.
That's a good one Jones. These are some of the key events that happen with the individual. These factors and the others I highlighted earlier (those to do with the test performance) will affect your ELISA.
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